Quantitative Tumorigenicity Assays Using the Anterior Chamber of the Mouse Eye1

Using a quantitative technic for depositing a definite number of carcinoma or sarcoma cells into the anterior chamber of the mouse eye, without leakage as proven by complete reten tion of Trypan blue dye, this route generally required a 100to 1,000-fold lower dose of cells to produce tumors in 100% of animals, as compared to the intramuscular, intravenous, and intraperitoneal routes of inoculation in both irradiated and nonirradiated mice. Neoplastic transformation of a cultured mouse cell line was demonstrated using the quantitative an terior chamber technic.